f zhang (Addgene inc)
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f zhang
F Zhang, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/f+zhang/pX601-AAV-CMV%3A%3ANLS-SaCas9-NLS-3xHA-bGHpA%3BU6%3A%3ABsaI-sgRNA+(Plasmid+%2361591)/pm41680487-183-15-17
Average 96 stars, based on 250 article reviews
F Zhang, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/f+zhang/pX601-AAV-CMV%3A%3ANLS-SaCas9-NLS-3xHA-bGHpA%3BU6%3A%3ABsaI-sgRNA+(Plasmid+%2361591)/pm41680487-183-15-17
Average 96 stars, based on 250 article reviews
f zhang - by Bioz Stars,
2026-09
96/100 stars
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Plasmid Preparation:Article Title: Conversion of IscB and Cas9 into RNA-guided RNA-editors Article Snippet: SaCas9 with sgRNA was a gift from Y. Huang (Addgene plasmids 99735) 79 . .. Dead PspCas13b with ADAR2dd (E488Q/T375G) was a gift from Article Title: Rules of engagement for condensins and cohesins guide mitotic chromosome formation. Article Snippet: .. Plasmids encoding a guide RNA and hCas9 were assembled by inserting double-stranded oligos into the pX330-U6-Chimeric_BB-CBh-hSpCas9 plasmid, which was a gift from Article Title: Modular engineering of thermoresponsive allosteric proteins. Article Snippet: The Renilla luciferase expression plasmid was purchased from Promega. .. H2B–GFP was a gift from G. Wahl (Addgene, plasmid 11680). pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA was a gift from Article Title: Rab10 inactivation promotes AMPAR trafficking and spine enlargement during long-term potentiation Article Snippet: SEPGluA1 was a gift from Dr. Scott Soderling at Duke University (Wang et al., 2008). mTurquoise2pBAD and mVenus- pBAD were gifts from Michael Davidson (Addgene plasmid # 54844 and # 54845). .. The human codon- optimized S. pyogenes Cas9 (SpCas9) and single guide RNA (sgRNA) expression plasmid was a gift from Article Title: Rab10 inactivation promotes AMPAR trafficking and spine enlargement during long-term potentiation Article Snippet: SEP-GluA1 was a gift from Dr. Scott Soderling at Duke University ( ). mTurquoise2-pBAD and mVenus-pBAD were gifts from Michael Davidson (Addgene plasmid # 54844 and # 54845). .. The human codon-optimized S. pyogenes Cas9 (SpCas9) and single guide RNA (sgRNA) expression plasmid was a gift from Article Title: Site-directed multivalent conjugation of antibodies to ubiquitinated payloads. Article Snippet: The oligos were phosphorylated with T4 PNK enzyme by incubation at 37 °C for 30 min and annealed by incubation at 95 °C for 5 min, followed by gradual cooling to 25 °C using a thermocycler. .. The annealed oligos were cloned into the plasmid pSpCas9(BB)-2A-Puro (PX459), which was obtained as gifts from Article Title: Site-directed multivalent conjugation of antibodies to ubiquitinated payloads Article Snippet: The oligos were phosphorylated with T4 PNK enzyme by incubation at 37 °C for 30 min and annealed by incubation at 95 °C for 5 min, followed by gradual cooling to 25 °C using a thermocycler. .. The annealed oligos were cloned into the plasmid pSpCas9(BB)-2A-Puro (PX459), which was obtained as gifts from Luciferase:Article Title: Modular engineering of thermoresponsive allosteric proteins. Article Snippet: The Renilla luciferase expression plasmid was purchased from Promega. .. H2B–GFP was a gift from G. Wahl (Addgene, plasmid 11680). pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA was a gift from Expressing:Article Title: Rab10 inactivation promotes AMPAR trafficking and spine enlargement during long-term potentiation Article Snippet: SEPGluA1 was a gift from Dr. Scott Soderling at Duke University (Wang et al., 2008). mTurquoise2pBAD and mVenus- pBAD were gifts from Michael Davidson (Addgene plasmid # 54844 and # 54845). .. The human codon- optimized S. pyogenes Cas9 (SpCas9) and single guide RNA (sgRNA) expression plasmid was a gift from Article Title: Rab10 inactivation promotes AMPAR trafficking and spine enlargement during long-term potentiation Article Snippet: SEP-GluA1 was a gift from Dr. Scott Soderling at Duke University ( ). mTurquoise2-pBAD and mVenus-pBAD were gifts from Michael Davidson (Addgene plasmid # 54844 and # 54845). .. The human codon-optimized S. pyogenes Cas9 (SpCas9) and single guide RNA (sgRNA) expression plasmid was a gift from Clone Assay:Article Title: Site-directed multivalent conjugation of antibodies to ubiquitinated payloads. Article Snippet: The oligos were phosphorylated with T4 PNK enzyme by incubation at 37 °C for 30 min and annealed by incubation at 95 °C for 5 min, followed by gradual cooling to 25 °C using a thermocycler. .. The annealed oligos were cloned into the plasmid pSpCas9(BB)-2A-Puro (PX459), which was obtained as gifts from Article Title: Site-directed multivalent conjugation of antibodies to ubiquitinated payloads Article Snippet: The oligos were phosphorylated with T4 PNK enzyme by incubation at 37 °C for 30 min and annealed by incubation at 95 °C for 5 min, followed by gradual cooling to 25 °C using a thermocycler. .. The annealed oligos were cloned into the plasmid pSpCas9(BB)-2A-Puro (PX459), which was obtained as gifts from |